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KMID : 0381120220440081017
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Genes and Genomics
2022 Volume.44 No. 8 p.1017 ~ p.1029
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MicroRNA-183 accelerates the proliferation of hepatocyte during liver regeneration through targeting programmed cell death protein 6
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Hou Fangxing
Li Xing
Wang Yanfeng
Xiao Xiangzuo
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Abstract
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Background: Liver regeneration is a highly orchestrated process concerning the modulation of various microRNAs (miRs). miR-183 was recently found to be involved in the process of liver regeneration, that miR-183 was remarkably up-regulated at 2?6 h after partial hepatectomy.
Objective: This study was aimed to explore the mechanism of miR-183 in on liver regeneration.
Methods: After partial hepatectomy (PH) or transfection, we measured the changes of miR-183 and programmed cell death protein 6 (PDCD6) levels in rats and the hepatocytes. The histopathology was observed with hematoxylin?eosin staining. The miR-183 mimic and inhibitor plasmids were intravenously injected into rats, and the liver weight/body weight ratio was calculated. The prediction of TargetScan and the validation of luciferase activity assay were employed to confirm the targeting relationship between miR-183 and PDCD6. The viability, apoptosis and cell cycle of transfected rat hepatocyte BRL-3A were determined via MTT and flow cytometry assays.
Results: MiR-183 expression showed a contrary tendency with that of PDCD6 during liver regeneration. Enhanced miR-183 in rats could notably increase liver/body weight ratio, while its inhibition did conversely. Overexpressed PDCD6, a target of miR-183, repressed the viability and cell cycle in hepatocytes, whereas its silence led to contrary results. Overexpressed miR-183 in BRL-3A cells enhanced cell viability and promoted the cell cycle yet suppressed apoptosis, whereas its inhibition showed contrary results, which were offset by PDCD6.
Conclusions: Collectively, miR-183 promoted liver regeneration via targeting PDCD6.
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KEYWORD
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Liver regeneration, Hepatocytes, Partial hepatectomy, Hepatic index, microRNA-183
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